Biochar is charcoal that is produced by pyrolysis of biomass in the absence of oxygen. Biochar can sequester atmospheric CO2, by doing so, contribute to goal of reducing global climate change. Poultry litter biochar is the black solid remaining after the pyrolysis of poultry litter. It has a higher cation exchange capacity (CEC). In this studying, different PL biochar samples are prepared at different production conditions such as temperature, different source of PL, different bedding. A few key properties of the biochars such as pH, water holding capacity, bulk density, and pore volume, surface area were measured as well as their chemical contents. After that, they were being used as adsorbent for removal of Pb2+ form aqueous solutions. Fixed-bed column studies were performed. Adsorption kinetics was analyzed using pseudo-second-order kinetic model and the Freundlich isotherm equation. How the biochar property affect the final adsorption capacity was studied. Optimized process for PL biochar production for Pb2+ elimination was suggested.